Reconstitution Calculator

Reconstitution, solved

Lyophilised vials arrive dry. This works out what the solution becomes once you add diluent — its concentration, the volume of one draw, and where that lands on an insulin syringe.

Inputs

Optional — fills in the vial strength for you.

Total peptide in the vial, in milligrams.

mg

How much peptide each draw should contain.

mcg

The diluent volume you reconstitute the vial with.

mL

Syringe size

Sets the scale on the barrel below.

Result

Live

Draw to

10units on a U-100 syringe

0255075100u
Concentration
5 mg/mL
Peptide per millilitre of solution
Draw volume
0.1 mL
1 mL = 100 units = 1 cc
Draws per vial
20
Whole draws at this amount
Per syringe unit
50 mcg
Peptide in each 0.01 mL graduation

Reference arithmetic only. Verify every figure independently before relying on it. VAULT products are supplied for laboratory research use and are not for human or veterinary administration — nothing here is dosing guidance.

How the arithmetic works

Everything above comes from one number: concentration. Divide the peptide in the vial by the volume of bacteriostatic water you add, and you have milligrams per millilitre. Every other figure follows from it.

  • Concentration = vial strength ÷ diluent volume
  • Draw volume = amount wanted ÷ concentration
  • Syringe units = draw volume × 100
  • Draws per vial = vial strength ÷ amount wanted

The diluent volume is a free choice, and it is a trade-off rather than a correct answer. Less water gives a concentrated solution where a draw is only a unit or two — quick, but a small misread costs proportionally more. More water spreads the same peptide across a longer stretch of the barrel, which is easier to read accurately and uses more of the vial's shelf life once it is wet.

Worked example

Vial strength
10 mg of lyophilised peptide
Diluent added
2 mL of bacteriostatic water
Concentration
10 ÷ 2 = 5 mg/mL
Amount wanted
500 mcg, i.e. 0.5 mg
Draw volume
0.5 ÷ 5 = 0.1 mL
On the barrel
0.1 mL × 100 = 10 units

Reference conversions: 1 mL = 100 units = 1 cc · 1 unit = 0.01 mL · 1 mg = 1000 mcg.